FIG. 5.

Mutation of Z residue 186 affects its ability to activate methylated Rp in CAT reporter gene assays. A vector containing the CAT gene linked to the BRLF1 promoter (Rp-CAT), either methylated or mock-methylated in vitro, was transfected into DG75 cells (A) or 293T cells (B) in combination with either empty vector (vector) or vector expressing wild-type Z (Wt Z), Z(S186A), or Z(S186T), as indicated. CAT activity was measured as the percentage of acetylation. The methylation status of each promoter construct is indicated. +, present; −, absent.