FIG. 7.

The Z(S186A) mutant is defective for inducing lytic EBV gene expression regardless of the methylation state of the EBV genome. 293/EBV-ZKO cells (untreated or pretreated with 5-aza-2′-dc, as indicated) were transfected with empty vector (vector) or a vector expressing wild-type Z (Wt Z, 0.5 μg in lane 2 and 1 μg in lane 3), Z(S186A), R, or both Z(S186A) and R, and immunoblots were performed for Z, R, BMRF1, or β-actin, as indicated. The methylation status of the EBV genome is indicated. +, present; −, absent.