FIG. 3.

Detection of B5 mRNA. Northern blot analyses to detect the presence of hfl-B5 mRNA. (A) Total RNA from the indicated cell line or (B) commercially available polyadenylated RNA from primary human fetal tissues or adult lung tissue probed with a DIG-11-dUTP-labeled hfl-B5 or β-actin-specific sequence. In panel A, the porcine cells are Seo vector only or CB5 that expresses myc-tagged B5. CB5 mRNA runs slightly larger due to the added epitope tags. The presence and quality of mRNA from all the cell lines were monitored by RT-PCR amplification of β-actin (not shown). In panel B, probes of the same primary tissue blots are shown with a B5-specific sequence (top) or a β-actin sequence (bottom).