Fig. 5.

Expression of CRH and its receptors and POMC and β-endorphin in inflamed tissue. CRH mRNA in inflamed paw was assessed with RT-PCR (A) 6 h following the injection of CFA (Lane 1, inflamed paw from Crh + / + mice; lane 2, inflamed paw from Crh-/- mice; lane 3, brain from Crh + / + mice; lanes 4 and 5, No-RT samples and lane 6, negative control). B CRFR1 and CRFR2 mRNA expression in inflamed paw was assessed 6 h following CFA injection (*:P = 0.05 between mice of the two genotypes under the same condition n = 3 mice/genotype/treatment; The test used was Mann–Whitney for comparison of each receptor mRNA expression between the two genotypes). Both receptors’ mRNA expression was normalized with the corresponding β-actin mRNA expression. POMC mRNA expression in inflamed paw was assessed with Real Time PCR (C) 6 h and (D) 24 h, following the injection with CFA. (*: P = 0.028, between mice of the two genotypes. n = 4 mice/genotype/treatment; ^$:P = 0.004 between Crh-/- mice exposed to the different treatment. n = 4 mice/genotype/treatment/experiment; #:P = 0.020 between Crh + / + and Crh-/-cort mice. The tests used were either Mann–Whitney or Kruskal–Wallis, followed by Dunn’s multiple comparison test). POMC mRNA expression was normalized with the corresponding β-actin mRNA expression. (E) β-endorphin protein levels in inflamed paw, as measured with ELISA, 24 h after CFA injection (n = 4 mice/genotype; Mann–Whitney). All data are expressed as median (min to max)