TABLE 3.
Site-directed mutagenesis primers
| Primer or primer pair | Sequencea (5′ to 3′) | Positionb (nt) | Mutation |
|---|---|---|---|
| NV G6855T, T6856A | CAGCTGCCGATCAATGGGTACTTTGTCTTTGTTTCATG | 6837-6874 | V500Y |
| NV C6855A, A6856T | CATGAAACAAAGACAAAGTACCCATTGATCGGCAGCTG | 6837-6874 | V500Y |
| NV A6897G, A6898C | GTGTCCAGATTTTATCAATTAGCGCCTGTGGGAACTGCCAGCTC | 6876-6919 | K514A |
| NV T6897C, T6898G | GAGCTGGCAGTTCCCACAGGCGCTAATTGATAAAATCTGGACAC | 6876-6919 | K514A |
| NV G6916A | GCCTGTGGGAACTGCCAACTCGGCAAGAGGTAGGC | 6899-6933 | S520N |
| NV C6916T | GCCTACCTCTTGCCGAGTTGGCAGTTCCCACAGGC | 6899-6933 | S520N |
| NV A6989G | CCAGATTTTATCAATTAAGGCCTGTGGGAACTGCCAG | 6880-6916 | K514R |
| NV T6898C | CTGGCAGTTCCCACAGGCCTTAATTGATAAAATCTGG | 6880-6916 | K514R |
| NV A6773C | GTCGGAATCTTGGGGACTTCAAAGCATACCCTG | 6757-6789 | E472D |
| NV T6773G | CAGGGTTAGCTTTGAAGTCCCCAAGATTCCGAC | 6757-6789 | E472D |
| NV A6772C | CGGTCGGAATCTTGGGGCATTCAAAGCATACCCTG | 6755-6789 | E472A |
| NV T6772G | CAGGGTATGCTTTGAATGCCCCAAGATTCCGACCG | 6755-6789 | E472A |
a
Mutant nucleotides shown in bold.
b
NV accession number M87661 in GenBank.