FIG. 4.

Western blot analysis of the H and F proteins of the parental and recombinant viruses. Vero cells were infected with a MOI of 0.01 and harvested when CPE was advanced. Proteins were separated by reducing SDS-polyacrylamide gel electrophoresis (7.5% for the H protein; 10% for the F protein) and blotted onto polyvinylidene difluoride membranes. The membranes were incubated with the anti-Fcyt rabbit antipeptide serum to detect the F proteins or with a mixture of the anti-Hcyt and anti-CDV-Hcyt rabbit antipeptide serum, respectively, to detect the H proteins.