FIG. 2.

Binding of HSV to the cell surface in the presence of various agents. HaCaT cells were treated for 1 h at 37°C with 30 μM monensin, 50 mM ammonium chloride, 100 nM bafilomycin A1 (BFLA), 200 μM genistein or 1 μg/ml heparin. ³⁵S-labeled HSV-1 KOS was added (MOI of 3) in the continued presence of agents for 1 h at 4°C. Cells were washed with PBS, detergent lysates were prepared, and samples were analyzed with a liquid scintillation counter. Cell-associated cpm represents virus that was bound to the cell surface. The mean of three replicate samples ± standard error is shown.