FIG. 4.

Colocalization of incoming HSV with dextran, a marker of fluid-phase endocytosis. HaCaT (A, B) or SK-N-SH (C) cells were chilled for 15 min, and then HSV-1 KOS K26GFP was added for 2 h at 4°C. Cells were washed, and then warmed medium containing 1 mg/ml 70,000-kDa dextran-Texas red was added, and cells were rapidly warmed to 37°C. At 12 min (A, C) or 45 min (B) p.i., cells were fixed with 3% paraformaldehyde. A confocal slice of a single cell is shown for each. The inset in panel A is a magnification of the boxed area. Bar, 5 μm.