FIG. 1.

Northern blot of RANTES mRNA in RSV-infected A549 cells. A549 cells were infected with RSV (MOI of 1) for various lengths of time (lane 1, uninfected cells; lane 2, 3 h postinfection; lane 3, 6 h postinfection; lane 4, 12 h postinfection; lane 5, 24 h postinfection). Total RNA was extracted from control and infected cells, and 20 μg of RNA was fractionated on a 1.2% agarose-formaldehyde gel, transferred to a nylon membrane, and hybridized to a radiolabeled RANTES or β-actin cDNA probe.