FIG. 3.

RANTES promoter activation following RSV infection. (A) Time course. A549 cells were transiently transfected with pGL2-974 and infected with RSV (MOI of 1). At different times postinfection, cells were harvested to measure luciferase activity. Uninfected plates served as controls. For each plate, luciferase activity was normalized to β-galactosidase reporter activity. Data are expressed as mean ± standard deviation of normalized luciferase activity. ∗, P < 0.01 relative to mock-infected plates. (B) Effects of different MOIs. A549 cells were transiently transfected with pGL2-974 and infected with RSV at MOIs of 0.1, 1, 3, and 10; 24 h after infection, cells were harvested to measure luciferase activity. Uninfected plates served as controls. For each plate, luciferase activity was normalized to β-galactosidase reporter activity. Data are expressed as mean ± standard deviation of normalized luciferase activity. ∗, P < 0.01 relative to mock-infected plates.