FIG. 4.

Effects of 5′ deletions and site mutations in the RANTES promoter sequence on RSV-inducible activity. (A) A549 cells were transiently transfected with 5′ deletions of the human RANTES promoter and infected with RSV for 24 h at an MOI of 1. Uninfected plates served as controls. For each plate, luciferase activity was normalized to β-galactosidase reporter activity. Data are expressed as mean ± standard deviation of normalized luciferase activity. ∗, P < 0.01 from pGL2-974. (B) A549 cells were transiently transfected with site-mutated (MUT) plasmids of the pGL2-220 RANTES promoter and infected with RSV for 24 h. Uninfected plates served as controls. For each plate, luciferase activity was normalized to β-galactosidase reporter activity. Data are expressed as mean ± standard deviation of normalized luciferase activity. ∗, P < 0.01 relative to -220 wild type (WT).