FIG. 7.

Effects of overexpressing DN mutant c-Jun and IRF transcription factors on RSV-induced RANTES gene transcription. 293 cells were transfected with 2 μg of pGL2-220 plasmid alone or cotransfected with either 0.5 μg of c-Jun DN or the empty vector pCMV (A) or with 0.2 μg of IRF-1, -3, and -7 DN or the corresponding empty vector (pCMV-BL for IRF-1 and -3; pCMV2 for IRF-7) (B). Transfected cells were infected with RSV (MOI of 1) and harvested at 24 h postinfection for determination of luciferase activity. Data are expressed as mean ± standard deviation of normalized luciferase activity. ∗, P < 0.01 relative to empty vector.