TABLE 1.
Cytokine concentrations in supernatants of VV-infected or uninfected keratinocytes and HMVEC-d culturesb
| Cytokine | Concn in supernatant (pg/ml)
|
|||
|---|---|---|---|---|
| Keratinocytes
|
HMVEC-d
|
|||
| Infected | Uninfected | Infected | Uninfected | |
| IL-1β | 5.9 | 5.6 | 3.8 | 3.7 |
| IL-2 | 7.8 | <3a | <3 | <3 |
| IL-4 | <3 | <3 | <3 | <3 |
| IL-5 | 6.5 | <5 | <5 | <5 |
| IL-6 | 41.4 | <5 | 12.5 | 47.9 |
| IL-8 | 121.1 | 50.4 | 744.7 | 1,676.9 |
| IL-12 p40 | 225.8 | <100 | <100 | <100 |
| IL-12 p70 | 29.2 | 22.7 | 21.6 | 27 |
| IFN-γ | <3 | <3 | <3 | <3 |
| IP10 | <30 | 64.3 | <30 | <30 |
| TNF-α | 17.5 | 5.4 | 10.5 | 15.5 |
a
Less than the detection threshold for the given cytokine.
b
Keratinocytes and HMVEC-d were either untreated or infected with VV at an MOI of 10 and cultured at 37°C for 24 h. Cytokine concentrations (pg/ml) in the supernatant samples were measured by MicroSpot ELISA array as described in Materials and Methods.