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Levels of rWT and mutant NS1 TX/98 proteins determined by metabolic labeling and Western blotting. (A) Metabolic labeling with [35S]Met-Cys of PK-15 cells infected with the rWT and deleted mutants (MOI = 2), from 8 to 10 h p.i. (B) Immunoblot with antibodies specific for NS1 and NP in PK-15 cells infected with rWT and the different mutants at 10 h p.i.
