Fig. 2.

Stability of the supercooled state of different static solutions. (A) Freezing assessment of clinical preservation solutions in vials using visual observation of ice formation shows Glycerol 5% in the VCA supercooling storage solution reached the lowest temperatures before freezing. However, these conditions were not statistically significant using the Mantel-Cox test for significance (n = 21–23 per condition). (B) Additions to HTK had no major differences in freezing temperatures between groups. Nonetheless, the groups with additions showed first signs of freezing at higher temperatures and had extended temperature ranges at which freezing occurred (n = 13 per condition).