Fig. 4.

Continuous visual temperature tracking using FLIR imaging. Non-invasive thermal imaging and (Bottom) representative clinical images show the VCA at all stages of the supercooling protocol. (A) During surgery, the vessels are the warmest structures, while the foot shows cooler temperatures after blood flow has been cut off. (B) During the loading phase, the temperature of the VCA is decreased, after which it is added to a (C-i) bag with CPAs at 4 °C. (C-ii) After supercooling, the bag with VCA is at -4 °C. (C-iii) Here, the VCA is shown in the bag after 1.5 min in the water bath. (D) The beginning and (E) end of the recovery phase show reheating of the VCA to 21 °C.