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. 2024 Sep 27;14:22339. doi: 10.1038/s41598-024-73549-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 6 — Perfusion parameters during the recovery phase. (A) Vascular resistance decreased faster in the stepwise groups (B) Potassium and (C) lactate level decreased over the course of recovery in all groups. (D) Glucose uptake showed an increase in the stepwise Glycerol 5% and EG group, with no significance. (E) Oxygen consumption was highest in the stepwise Glycerol 5% BSA15% group at 90 min compared to the single-step group (°) and the stepwise Glycerol 5% group (*). (F) Initial weight is equivalent to 0%. Weight gain at the end of the recovery phase was highest in the stepwise EG and single-step Glycerol group. **/°° p ≤ 0.0021; ***/°°° p ≤ 0.0002; **** p ≤ 0.0001.