TABLE 3.
Postchallenge neutralizing antibody titers
Wk | 50% Neutralization titer for strain and macaque no.a:
|
|||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mm 12-94b
|
Mm 169-84b
|
Mm 245-91b
|
Mm 415-90b
|
Mm 95-00b
|
Mm 232-95b
|
|||||||||||||
239 | 316 | 251LA | 239 | 316 | 251LA | 239 | 316 | 251LA | 239 | 316 | 251LA | 239 | 316 | 251LA | 239 | 316 | 251LA | |
0 | — | 30 | 80 | — | 80 | 120 | — | 40 | 60 | — | 60 | 80 | — | — | — | — | — | — |
2 | — | 480 | 1,920 | — | — | 160 | — | 120 | 240 | — | 80 | 240 | — | — | — | — | — | — |
4 | — | NT | 7,680 | — | NT | 5,120 | — | NT | 5,120 | — | NT | 7,680 | — | NT | 160 | — | NT | 160 |
20 | — | NT | 10,240 | — | NT | 7,680 | — | NT | 15,360 | 20 | NT | 1,920 | 60 | NT | 5,120 | 20 | NT | 3,840 |
Neutralizing antibody titers indicate the reciprocal of the plasma dilution that reduced SIV infection of CEMx174 cells containing a Tat-inducible SEAP reporter construct by 50%. Antibody neutralization was tested against SIVmac239 (239), SIVmac316 (316), and a lab-adapted strain of SIVmac251 (251LA). Samples that were negative for virus neutralization at the lowest dilution tested (<1:20) are indicate by a dash (—). Time points that were not tested for neutralization of SIVmac316 are indicated by NT.
Mm 12-94, Mm 169-84, Mm 245-91, and Mm 415-90 were immunized with three doses of scSIVmac239Nefsff and scSIVmac316Nefsff. Mm 95-00 and Mm 232-95 are unvaccinated, Mamu-A*01+ control animals. All six animals were challenged intravenously with SIVmac239 6 weeks after the third scSIV immunization.