Skip to main content
. 2005 Jun;79(12):7707–7720. doi: 10.1128/JVI.79.12.7707-7720.2005

TABLE 3.

Postchallenge neutralizing antibody titers

Wk 50% Neutralization titer for strain and macaque no.a:
Mm 12-94b
Mm 169-84b
Mm 245-91b
Mm 415-90b
Mm 95-00b
Mm 232-95b
239 316 251LA 239 316 251LA 239 316 251LA 239 316 251LA 239 316 251LA 239 316 251LA
0 30 80 80 120 40 60 60 80
2 480 1,920 160 120 240 80 240
4 NT 7,680 NT 5,120 NT 5,120 NT 7,680 NT 160 NT 160
20 NT 10,240 NT 7,680 NT 15,360 20 NT 1,920 60 NT 5,120 20 NT 3,840
a

Neutralizing antibody titers indicate the reciprocal of the plasma dilution that reduced SIV infection of CEMx174 cells containing a Tat-inducible SEAP reporter construct by 50%. Antibody neutralization was tested against SIVmac239 (239), SIVmac316 (316), and a lab-adapted strain of SIVmac251 (251LA). Samples that were negative for virus neutralization at the lowest dilution tested (<1:20) are indicate by a dash (—). Time points that were not tested for neutralization of SIVmac316 are indicated by NT.

b

Mm 12-94, Mm 169-84, Mm 245-91, and Mm 415-90 were immunized with three doses of scSIVmac239Nefsff and scSIVmac316Nefsff. Mm 95-00 and Mm 232-95 are unvaccinated, Mamu-A*01+ control animals. All six animals were challenged intravenously with SIVmac239 6 weeks after the third scSIV immunization.

HHS Vulnerability Disclosure