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. 2024 Sep 27;14:22399. doi: 10.1038/s41598-024-73283-1

Fig. 2.

Fig. 2

CRISPR-CAS9 knockdown of Tyrp1. Melanin pigmentation was greatly reduced in F0 Tyrp1 crispant larvae relative to F0 non-injected control siblings. F0 Tyrp1 crispant electropherograms showed overlapping sequence traces at the gRNA target site for forward and reverse DNA sequencing reactions, consistent with genome editing. F0 non-injected control DNA sequence does not show evidence of genome editing. The gRNA target sequence (underlined) overlaps the deleted nucleotide in the copper Tyrp1 allele.