TABLE 1.
Oligonucleotides used in PCR and mutagenesis
| Name | Sequence (5′-3′) |
|---|---|
| 3DFORBGL | CCAGATCTGGGTTGATTGTGGACACCAGAGAT |
| 3DREVSPH | CCGCATGCCGCGTCACCGCACACGGCGTTCAC |
| 3CFORBGL | CCAGATCTAGTGGTGCCCCACCGACCGACTTG |
| C163FOR | CACCAAGGCTGGTTACGGCGGAGGAGCCGTTC |
| C163REV | GAACGGCTCCTCCGCCGTAACCAGCCTTGGTG |
| SacIImutFor | CAGGGTAAACGCCGTGGCGCGCTCATCGAC |
| SacIImutRev | GTCGATGAGCGCGCCACGGCGTTTACCCTG |
| Ub3DpolSacII | GCGCCGCGGTGGAGGGTTGATTGTGGACACCAGA |
| Ub3DpolBgIII | GCGAGATCTTTACGCGTCACCGCACACGGCGTT |
| GC-cremut-FOR(A1-C) | GAGGAGGACTTGTACCAACACGATCTAAACAG |
| GC-cremut-REV(A1-C) | CTGTTTAGATCGTGTTGGTACAAGTCCTCCTC |
| GC-cremut-FOR(A2-C) | GAGGAGGACTTGTACACACACGATCTAAACAG |
| GC-cremut-REV(A2-C) | CTGTTTAGATCGTGTGTGTACAAGTCCTCCTC |
| GC-cremut-FOR(A3-C) | GAGGAGGACTTGTACAACCACGATCTAAACAG |
| GC-cremut-REV(A3-C) | CTGTTTAGATCGTGGTTGTACAAGTCCTCCTC |
| GC-cremut-FOR(A1A2-C) | GAGGAGGACTTGTACCCACACGATCTAAACAG |
| GC-cremut-REV(A1A2-C) | CTGTTTAGATCGTGTGGGTACAAGTCCTCCTC |
| T7crefor | AATTCTAATACGACTCACTATAGGGTCGCTTGAGGAGGACTTGTACAAACACGATCTAAACAGGTTTCCCCAACTGACCCG |
| T7crerev | CTAGCGGGTCAGTTGGGGAAACCTGTTTAGATCGTGTTTGTACAAGTCCTCCTCAAGCGACCCTATAGTGAGTCGTATTAG |
| 01Kcremut FOR(A1C) | GAGGAGGACTTGTACCAACACGATCTATGCAG |
| 01Kcremut-REV(A1C) | CTGCATAGATCGTGTTGGTACAAGTCCTCCTC |
| 01Kcremut-FOR(A2C) | GAGGAGGACTTGTACACACACGATCTATGCAG |
| 01Kcremut-REV(A2C) | CTGCATAGATCGTGTGTGTACAAGTCCTCCTC |
| 01Kcremut-FOR(A3C) | GAGGAGGACTTGTACAACCACGATCTATGCAG |
| 01Kcremut-REV(A3C) | CTGCATAGATCGTGGTTGTACAAGTCCTCCTC |
| F-O1KcreDEL | GATTGTACTGAGAGTGCACCATATCGACGC |
| R-O1KcreDEL | GTTTCACAGTAAAGCTGCCGTGCTGGGGTT |
| F-O1KcreDEL-overlap | TTGAGGAGGACTTGTACCACGATCTATGCAGGTTTC |
| R-O1KcreDEL-overlap | GAAACCTGCATAGATCGTGGTACAAGTCCTCCTCAA |