TABLE 4.
Virus isolation and RT-PCR of tissuesa
| Sample | Result on dpi for pig no.:
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 3 dpi
|
4 dpi
|
5 dpi
|
6 dpi
|
7 dpi
|
8 dpi, 4 | ||||||
| 3 | 21 | 6 | 18 | 2 | 20 | 5 | 17 | 1 | 19 | ||
| Hindbrain | − | − | 7.7 | − | − | + | |||||
| Forebrain | − | − | 6.9 | − | − | + | |||||
| Trigeminal ganglion | − | − | − | − | 2.1 | 2.4 | 5.5 | − | 2.1 | 3.3 | + |
| Pons | + | + | + | − | 4.3 | ||||||
| Thalamus | + | − | + | − | 4.1 | ||||||
| Cerebellum | − | − | + | + | 2.9 | ||||||
| Olfactory bulb | + | + | − | 3.6 | 2.4 | ||||||
| Optic nerve | − | − | − | − | 4.4 | ||||||
| Medulla oblongata | − | − | − | − | 4.0 | ||||||
| Midbrain | − | − | 3.3 | − | 4.0 | ||||||
| Basal ganglia | − | − | 2.1 | − | 2.4 | ||||||
| Meninges | ND | − | ND | ND | 4.4 | ||||||
| CSF | + | + | + | + | 4.7 | ||||||
| Lung | − | − | − | 2.1 | 2.8 | − | − | 3.4 | 2.4 | − | − |
| Bronchus | − | 4.4 | − | + | − | ||||||
| Trachea | 3.5 | + | 3.4 | + | 5.2 | 2.4 | − | 3.3 | − | 3.7 | − |
| Nasal turbinates | 5.0 | 4.1 | 2.6 | 4.1 | 5.3 | 4.3 | − | 4.4 | − | 3.7 | + |
| Tongue | − | 2.1 | 2.1 | 2.6 | − | ||||||
| Submandibular lymph node | 2.6 | − | 3.1 | − | 5.8 | − | 5.9 | 3.5 | 6.0 | 3.4 | + |
| Bronchial lymph node | − | (+) | − | + | − | 3.9 | 2.9 | 4.0 | − | 4.0 | − |
| Conjuctival lymphoid tissue | + | + | + | + | + | ||||||
| Retrobulbar lymphoid tissue | (+) | (+) | + | + | 3.3 | ||||||
| Tonsil | − | − | − | − | − | − | − | − | − | 4.3 | − |
| Spleen | − | + | − | + | − | 2.1 | − | 2.1 | − | + | − |
a
See Table 1, footnote a.