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. 2024 Sep 27;11:1040. doi: 10.1038/s41597-024-03880-2

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2024

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Fig. 1 — Experimental workflow. PBMCs were collected from healthy donors and sorted into different cell populations. RNA was prepared and its quality and concentration assayed according to standardized techniques. A total of 100 ng per reaction were used as input material to amplify GPCR transcripts in eight separate reactions each containing 50 GPCR specific primer pairs and aminoallyl-dUTP. Separate reactions with 10 ng of input material were used to determine those receptors expressed at higher levels. PCR reactions were pooled and Cy5 labelled on the amino-modified nucleotides. Labelled PCR products were hybridized to custom GPCR arrays that contained plus and minus sense oligonucleotides. Arrays were processed and scanned, and their signal extracted analyzed.