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. 2024 Sep 27;11:1040. doi: 10.1038/s41597-024-03880-2

Fig. 3.

Fig. 3

GPCR expression detection method comparison. (A) Venn analysis was used to compare the sensitivity of our method compared to the Groot et al. study at a Ct threshold of 35 cycles. Us-assayed means the GPCRs assayed in our study. Us-detected, the GPCRs detected in our 100 ng input assay. Groot-assayed, the total amount of GPCRs assayed by Groot-Kormelink et al. Groot-35 the GPCRs detected by TaqMan QPCR assays at a 35-cycle threshold in that study. (B) Venn analysis was used to compare the sensitivity of our methods compared to the Groot et al. study at a Ct threshold of 32 cycles. Us-assayed, the GPCRs assayed in our study. Us-detected, the GPCRs detected in our 100 ng input assay. Groot-assayed, the total amount of GPCRs assayed by Groot-Kormelink et al. Groot-32 the GPCRs detected by TaqMan QPCR assays at a 32-cycle threshold in that study.