FIG. 6.

Western blots. A. Tissue specificity of the anti-P-vank-1 Ab in 3-day-p.p. insects. Crude protein extracts were prepared from nonparasitized control (C) and 3-day-parasitized (3-day) H. virescens whole-body tissue (WB), hemocytes (HC), nerve cord (NC), hemolymph plasma (PL), digestive tract (DT), epidermal layer (EP), and fat body tissue (FB). A 19-kDa protein of the predicted size from parasitized larval whole-body, epidermal layer, and fat-body extracts reacted specifically with the anti-P-vank-1 Ab. The signal appeared by 1 day p.p. in the fat body (B), and the Ab was sensitive to as little as 5 μg (C) of extract from the 3-day-p.p. fat body.