Table 1.
Assay parameters and method validation for the determination of Ipratropium and Fenoterol pure samples by the proposed spectrophotometric methods.
| Parameter | ICS method | IDW method | IAM method | ||
|---|---|---|---|---|---|
| IPR or FEN | IPR | FEN | IPR | FEN | |
| Wavelength (nm) | 220.0 | 210.0 & 220.0 | 210.0 & 220.0 | 210.0 | 210.0 |
| Linearity Range (µg/mL) | 2.0–40.0 | 5.0–40.0 | 2.0–40.0 | 5.0–40.0 | 2.0–40.0 |
| -Slope | 0.0487 | 0.0126 | 0.0238 | 0.9823 | 0.9063 |
| -SE of the slope | 0.0001 | 0.0001 | 0.0001 | 0.0110 | 0.0030 |
| -Intercept | 0.0185 | -0.0053 | 0.0022 | 0.7711 | 0.4124 |
| -SE of the intercept | 0.0027 | 0.0015 | 0.0032 | 0.2561 | 0.0657 |
| -Correlation coefficient (r) | 1.0000 | 0.9999 | 0.9999 | 0.9997 | 1.0000 |
|
Accuracy a (Mean ± RSD%) |
100.67 ± 1.289 |
100.27 ± 1.175 |
98.91 ± 0.825 |
100.04 ± 0.609 |
100.69 ± 0.989 |
| Robustness (RSD%) | 1.070 | 1.045 | 0.972 | 1.106 | 0.892 |
| Precision b (RSD%) | |||||
| -Repeatability | 0.797 | 0.644 | 0.973 | 0.432 | 0.358 |
| -Intermediate precision | 1.019 | 1.014 | 1.208 | 0.955 | 0.787 |
| LOD (µg/mL) | 0.225 | 0.408 | 0.555 | 0.880 | 0.300 |
| LOQ (µg/mL) | 0.683 | 1.236 | 1.682 | 2.666 | 0.909 |
aAverage of five determinations.
bAverage of three determinations.