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Complementation of the L-negative HBV genome by L derivatives. Cytoplasmic (upper panel) or secreted, virion-derived (lower panel) nucleocapsids were detected by the endogenous polymerase reaction. Single numbers correspond to the C-terminal ends of the deletions in L. 234-251, derivative of parental L expression plasmid (Fig. 1C) lacking codons 234 to 251 of the L open reading frame; −, transfection without L expression vector; P, complementation with the parental L expression vector.
