FIG. 1.
SV5-matured C57BL/6 BMDC are much more efficient inducers of T-cell proliferation than rSV5-matured BALB/c BMDC. (A) C57BL/6 BMDC infected with rSV5 or treated with LPS for 24 h were pulsed with 10−10 M Ova peptide and then added to CFSE-labeled, Ova257-264-specific TCR transgenic T cells for 3 days. (B) BALB/c BMDC infected with rSV5 or treated with LPS for 24 h were pulsed with 10−9 M listeria p60217-225 peptide and then added to CFSE-labeled, listeria p60-specific TCR transgenic T cells for 3 days. (C) Table showing the analysis from the proliferation assay of T cells. The proliferation index is the average number of divisions in cells that divided and the division index is the average number of divisions the cell population has undergone. The proliferation profiles shown are representative of three independent experiments. Note: the peptide concentration used was the minimal concentration of peptide that gave detectable proliferation of antigen-specific T cells with SV5-matured DC.