FIG. 2.

rSV5-infected C57BL/6 and BALB/c BMDC produce a similar subset of proinflammatory cytokines. In vitro-cultured BMDC were mock-infected, LPS-treated, and infected with rSV5 at an MOI of 2, 10, or 50 or with UV-inactivated rSV5 (MOI = 10). Twenty-four hours after infection, supernatants from the cultures were harvested and cytokines were measured using ELISA (IL-12p40) or cytometric bead analysis (TNF-α and IL-6). IFN-α/β was measured using a biological assay based on vesicular stomatitis virus (VSV)-mediated cytolysis of NTCC L929 cells. Poly(I:C) was used as a positive control for IFN-α/β production by BMDC. (A) IL-12p40, (B) IL-6, (C) IFN-α/β, (D) TNF-α. The graphs show the average ± standard error of the mean of four independent experiments.