FIG. 6.

Binding of secreted IFN-α/β to its receptor is required for the upregulation of CD40, CD80, and CD86. BMDC from IFN-α/βR^−/− and control mice (129S1/SvImJ) were mock infected, treated with LPS, or infected with rSV5. Twenty-four hours later, cells were stained for CD11c and CD40, CD80, or CD86 and analyzed using flow cytometry. The graphs in the figure show the geometric mean fluorescence intensity of CD40, CD86, and CD80 for CD11c⁺ cells. The graphs show the average ± standard error of the mean of three independent experiments.