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Interaction of APE1 with Kap α1 and α2 analyzed by FLAG-immunoprecipitation. HCT116 cells were transiently transfected with a vector control or with a plasmid DNA expressing C-terminally FLAG-tagged APE1. The extracts were processed with immunoprecipitation using FLAG antibody to enrich the APE1-FLAG protein, and were analyzed by western blotting using Kap α1 (A) or Kap α2 (B) antibody.
