Table 2.

Nuclear distribution of various EGFP fusion proteins

EGFP fusion proteins	Nuclear fraction (% of total)
EGFP	12.4 ± 3
WT APE1-EGFP	97.2 ± 3.6
N20-EGFP	54.9 ± 5.2
ND20 APE1-EGFP	40.5 ± 3.3
ND41 APE1-EGFP	32.3 ± 2.5
APE1(K6R/K7R)-EGFP	85.6 ± 6.4
APE1(K6L/K7L)-EGFP	87.2 ± 5
APE1(Lall)-EGFP	68.5 ± 8.2
ND7 APE1-EGFP	77.2 ± 6.6
ND7 APE1(V10A)-EGFP	75.3 ± 2.6
ND7 APE1(E12A/D13A)-EGFP	32.8 ± 8.1
Full-length APE1(E12A/D13A)-EGFP	74.5 ± 2.8
ND7-APE1(E12A/D13A)-EGFP+LMB	92.7 ± 2.5

Intensities of whole cells and of nuclei were measured and ratios of nuclear versus whole cell intensities were calculated from >30 individual cells in each experiment.