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PCR amplification of the three fragments of the TOP1, P53 and CYP1A2 genes in MDA products of 16 sperm aliquots treated using two different lysis methods. The fragments are 1080 bp in length for TOP1, 643 bp for P53 and 550 bp for CYP1A2. Eight aliquots from the dilution of 3 cells/3 μl were lysed on ice and the other eight were lysed at 65°C.
