Table 2.
Total copy number for nine genes (TOP1, P53, CYP1A1, C6orf195, ADCYAP1, PTEN, PMS2 and CAT) and the human DNA representative rate in each 50 μl MDA product
| S01 | S04 | S05 | S06 | S08 | S13 | S14 | S15 | S16 | S16 MDA2 | |
|---|---|---|---|---|---|---|---|---|---|---|
| TOP1 | 2.2E+6 ± 0.4E+6 | 2.1E+7 ± 0.1E+7 | 23.0E+6 ± 0.5E+6 | 1.6E+8 ± 0.2E+8 | 4.1E+3 ± 0.5E+3 | 250* | 2.6E+7 ± 0.2E+7 | 4.1E+3 ± 0.5E+3 | 250* | 0* |
| P53 | 2.4E+5 ± 0.5E+5 | 3.6E+5 ± 0.2E+5 | 6.1E+4 ± 0.5E+4 | 2.1E+6 ± 0.1E+6 | 0* | 250* | 2.1E+6 ± 0.1E+6 | 1.2E+7 ± 0.1E+7 | 2500* | 2500* |
| CYP1A1 | 5.5E+5 ± 1.1E+5 | 3.9E+7 ± 0.1E+7 | 3.9E+6 ± 0.7E+6 | 33.5E+4 ± 0.4E+4 | 2.1E+6 ± 0.2E+6 | 15.3E+4 ± 0.5E+4 | 7.1E+3 ± 2.5E+3 | 2500* | 3.4E+7 ± 0.1E+7 | 1.0E+7 ± 0.2E+7 |
| PIK3CA | 250* | 3.8E+5 ± 0.4E+5 | 4.1E+5 ± 0.6E+5 | 1.3E+7 ± 0.2E+7 | 2.9E+6 ± 0.6E+6 | 5.0E+3 ± 0.1E+3 | 0* | 1.0E+4 ± 1.0E+4 | 250* | 250* |
| C6orf195 | 5.8E+6 ± 0.3E+6 | 3.1E+5 ± 0.1E+5 | 8.9E+3 ± 0.3E+3 | 1.3E+6 ± 0.1E+6 | 6.1E+4 ± 1.7E+4 | 2500* | 3.2E+6 ± 0.5E+6 | 5.4E+5 ± 0.4E+5 | 4.2E+5 ± 0.5E+5 | 1.0E+5 ± 0.1E+5 |
| ADCYAP1 | 250* | 3.6E+6 ± 0.2E+6 | 8.8E+3 ± 3.6E+3 | 18.7E+5 ± 0.3E+5 | 0* | 2500* | 7.0E+5 ± 1.1E+5 | 2.4E+5 ± 0.1E+5 | 6.1E+5 ± 0.2E+5 | 2.0E+5 ± 0.2E+5 |
| PTEN | 13.7E+4 ± 0.6E+4 | 23.3E+4 ± 0.2E+4 | 5.6E+3 ± 4.5E+3 | 3.2E+6 ± 0.2E+6 | 4.8E+6 ± 0.6E+6 | 3.6E+6 ± 0.3E+6 | 4.4E+3 ± 0.7E+3 | 16.7E+6 ± 0.2E+6 | 5.8E+5 ± 0.2E+5 | 2.9E+5 ± 0.2E+5 |
| PMS2 | 2.6E+5 ± 0.2E+5 | 1.4E+6 ± 0.1E+6 | 2.1E+6 ± 0.1E+6 | 9.6E+5 ± 0.6E+5 | 1.6E+6 ± 0.4E+6 | 12.4E+5 ± 0.2E+5 | 19.7E+5 ± 0.2E+5 | 9.7E+5 ± 0.7E+5 | 4.5E+5 ± 0.1E+5 | 1.8E+5 ± 0.3E+5 |
| CAT | 250* | 1.6E+6 ± 0.1E+6 | 9.9E+3 ± 1.4E+3 | 5.0E+6 ± 0.4E+6 | 2.3E+4 ± 0.7E+4 | 2.8E+6 ± 0.4E+6 | 3.7E+6 ± 1.9E+6 | 5.1E+3 ± 0.6E+3 | 0* | 0* |
| Yield (μg) | 13 | 20 | 29 | 26 | 32 | 29 | 26 | 35 | 14 | 30 |
| A.C.N./ng | 80 | 379 | 114 | 774 | 40 | 30 | 158 | 98 | 295 | 41 |
| R.R. (%) | 29 | 135 | 41 | 276 | 14 | 11 | 56 | 35 | 105 | 15 |
Total copy number was displayed in mean ± SD format and the numbers are written in scientific format. A.C.N./ng is for average copy number per ng DNA and R.R. for human DNA representative rate. For those samples on which real-time PCR did not work (labeled with asterisk), 0, 250 and 2500 were separately assigned in accordance with no amplification, successful amplification in 1/5C0 and 1/50C0 MDA products displayed in Figure 3.