Fig. 4. Ly6a^high T cells are enriched in the tumor microenvironment independently of UVB exposure.

a Experimental flowchart. b Left: Representative flow cytometry analyzes of Ly6a expression levels in CD4⁺ (left) and CD8⁺ (right) T cells from inguinal lymph nodes, spleens, and tumors. Right: MFI of Ly6a normalized to isotype control (left) and percentage of Ly6a^high cells in total CD4⁺ or CD8⁺ compartments (right) (n = 5 per condition). c Violin plots of Ly6a expression in CD4⁺ (left) and CD8⁺ (right) cells in lymph nodes and tumors. Single-cell RNA-seq data from Davidson et al.³⁰. d Experimental flowchart: Melanoma metastases were isolated from mouse lungs, and CD45⁺ cells were isolated and analyzed using CyTOF. CD4⁺ cells and CD8⁺ cells are divided into three groups: CD62l⁺ (blue gate), Ly6a^high (red gate), and Ly6a^low (green gate). e Heatmap of median expression levels of T cell markers in indicated cells. The colors reflect the transformed ratio relative to the minimum expression of the marker (n = 4 per group). Statistical significance was determined by one-way ANOVA test with Tukey correction (b) or two-tailed t test (c). n.s. not significant. Error bars represent standard errors. Source data are provided as a Source Data file.