Fig. 3. Screening of 3.10C2 parallel lineage V_H clones.

Antibody 3.10C2 and antibodies formed by selected V_H6-8 variable regions and the 3.10C2 light chain were tested in ELISA with a peptide encompassing the entire TREM2 stalk region or peptides of the indicated TREM2 fragments or control inhibin 30-mer peptide. ELISA binding is indicated in red, with the optical density scale shown to the right. The CDR H3 sequences of V_H6-8 parallel clone candidates are shown to the right. V_H sequences belonging to the same clonotype (CDR H3 amino acid identity >67% for the same CDR H3 length) are indicated by letters next to the CDR H3 sequences. The relative number of read counts with V_H6-8 germline segments and the CDR H3 sequences of the hybridoma or selected clones in the deep sequencing datasets of rats R18, R19, and R20 are shown on the right in blue shading as indicated by the color scale and percentage shown in boxes. Zero values indicate less than 0.5% and blank values indicate no reads found. The maximum CDR H3 sequence identities among clones of the same CDR H3 length or different lengths with one or two contiguous gaps are indicated on the right. Inhibin refers to a 30-mer control peptide with the mouse inhibin sequence unrelated to TREM2. OD, A₄₅₀ ELISA optical density. Source data are provided as a Source Data file.