Table 1.

Binding kinetics and off-target binding potential of rat and humanized antibodies 3.10C2 and Para.09

Antibody	ka (M−1s−1)a	kd (s−1)a	KD (M)	BV ELISA scoree
3.10C2b	9.7 × 105 [1.31]c	1.9 × 10−4 [2.42]	2.0 × 10−10 [1.89]	0.73
Para.09b	2.2 × 106 [1.15]	4.4 × 10−5 [2.31]	2.0 × 10−11 [2.04]	0.66
hu3.10C2d	8.8 × 105 [1.13]	3.7 × 10−4 [1.07]	4.2 × 10−10 [1.10]	0.87
huPara.09d	2.6 × 106 [1.22]	5.6 × 10−5 [1.72]	2.2 × 10−11 [1.43]	0.99

^aSPR analysis performed at 37 °C with an 1-hour dissociation step with full TREM2 ECD.

^bChimeric rat/human IgG1 antibodies.

^cNumbers are geometric mean and fold-difference from geometric mean, within brackets, of n = 4 independent experiments.

^dLight chains derived from clonal variant 3.27H7.

^eScores below 5 are considered negative for off-target binding.