TABLE 2.
Efficiency of plus-strand DNA synthesis determined by primer extension
| Plus-strand eventa | % Efficiency (mean ± SD)b for:
|
||||||
|---|---|---|---|---|---|---|---|
| WT | DR1-13 | DR1-Xho | DR1-Pvu | DR1-12 | DR2-12 | DRs-12 | |
| Priming from DR2c | 77 ± 13 | 57 ± 1 | 65 ± 6 | 32 ± 5 | 49 ± 6 | 52 ± 12 | 55 ± 9 |
| Priming from DR2 and circularizationd | 55 ± 5 | 18 ± 4 | 15 ± 5 | 5 ± 2 | 3 ± 1 | 38 ± 9 | 7 ± 2 |
| Circularizatione | 73 ± 14 | 31 ± 7 | 23 ± 5 | 13 ± 5 | 7 ± 3 | 74 ± 6 | 13 ± 4 |
| In situ primingf | 5 ± 1 | 15 ± 4 | 16 ± 4 | 26 ± 3 | 22 ± 3 | 10 ± 3 | 21 ± 4 |
| Primer utilization totalg | 82 | 72 | 81 | 58 | 71 | 62 | 76 |
a
See Materials and Methods for explanation.
b
Values are based on three measurements made with intracellular viral DNA isolated from independent transfections, except for the wild-type (WT) and DRs-12 values, which are based on six measurements.
c
B(DR2)/M value.
d
A(DR2)/M value.
e
A(DR2)/B(DR2) value.
f
A(DR1)/M value.
g
Priming from DR2 plus in situ priming value.