FIG. 6.

Inhibiting eIF4E phosphorylation significantly reduces HCMV replication and the accumulation of late viral proteins in quiescent cells infected at low input doses of virus. (A) Primary human fibroblasts (NHDF cells) were growth arrested by serum starvation and infected (MOI = 0.05) in the presence or absence of the mnk inhibitor CGP57380. After 7 days, cell-free lysates were prepared by freeze-thawing, and the titer in NHDF cells was determined. (B) Cells were infected as described in the legend to panel A. After 7 days, total protein was isolated, fractionated by SDS-PAGE, and analyzed by immunoblotting using antisera directed against pp28 or actin.