FIG. 6.

Overexpression of an antisense NESI RNA blocked the expression of an exogenous His-tagged NESI in mammalian cells. COS7 cells were cotransfected with plasmid pEGFP-N1 encoding GFP, plasmid pcDNA-NESI-V5HisTopo encoding a His-tagged NESI, and various amounts of the effector plasmid pcDNA3.1-AS-NESI that expresses an antisense RNA of NESI as indicated. Two days posttransfection, protein lysates were prepared from the transfected cells and subjected to Western blot analysis using antibodies as indicated.