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Viral replication and protein expression of Cys-to-Ala mutants of the HXB2 strain. (A and B) Cell-free, WT, and Cys-to-Ala mutant viruses as indicated were assayed for viral replication kinetics in CEM-SS (A) and PM1 (B) cells. (C and D) HeLa (C) and 293T (D) cells were transfected with the WT or mutant proviruses, and cell and virion lysates were assessed by Western blot analysis.
