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Assessment of the effect of the Env/p24 ratio of the virus on viral infectivity. (A) 293T cells were transfected with 10 μg of WT pHXB2RU3 (lanes 1 and 3) or with 7.5 μg each of pHXBΔBglCAT and WT pSVE7puro (lanes 2 and 4), and cell and virion lysates were analyzed by Western blotting. (B) Viruses produced from transfection with each of the WT and mutant proviruses were normalized for RT and then used to challenge H938 cells. CAT activity was determined 2 days postinfection.
