FIG. 5.

Restriction enzyme analysis of strand transfer products produced by HIV-1 IN with the wt 4.1-kbp DNA donor. The strand transfer products were produced by IN at 5 nM (top). The integration products were divided into two nearly equal samples that were not (−) or were (+) digested by EcoRI. Lanes 1 and 2 were DNA donor without IN, while lanes 3 and 4 were with IN. Lane 5, the 1-kbp DNA ladder. The U3 and U5 half-site products (bold) and the 4.3-, 3.6-, and 2.9-kbp full-site products (bold) in lane 4 after EcoRI digestion are identified on the top right and bottom right, respectively. The positions of four DNA markers (not bold) are also illustrated on the right. The marker positioned between the 3.0- and 5.0-kbp markers is 4.0 kbp in length.