TABLE 1.

Comparison of the genomic and antigenomic RNA syntheses and mRNA transcription^a

Characteristic	Genomic synthesis (AG to G)	Antigenomic synthesis (G to AG)	mRNA transcription
Sensitivity to α-amanitin	Sensitive (inhibited at 1-5 μg/ml)	Resistant (inhibited at >100 μg/ml)	Sensitive (inhibited at 1-5 μg/ml)
S-HDAg requirement
S-177 phosphorylation	Yes	No	?
R-13 methylation	Yes	No	?
K-72 acetylation	Yes	?	?
Assisted by recombinant S-HDAg from E. coli	No	Yes	?
Cytoplasmic transport after synthesis	Yes	No	Yes
Inhibition by L-HDAg	Yes	No	?
Polymerases	pol II	pol I (?)	pol II
Site of synthesis	Nucleoplasm	Nucleolus	Nucleoplasm

^aAG, antigenomic RNA; G, genomic RNA.