FIG. 1.

SAPK activation is dependent on viral gene expression. Replicate CV-1 cultures were mock infected or infected with HFEM or tsB7 at 33, 37, or 39°C as indicated, and lysates were prepared at 8 h postinfection. Western blot analysis of proteins was as described in Materials and Methods. A representative of three independent experiments is shown. Band intensities of phospho-JNK (pJNK) and phospho-p38 (pp38) were quantified using Image J as described in Materials and Methods, and results are presented as a percentage of wild-type parental virus (HFEM) values below the corresponding tsB7 lanes (7-9).