FIG. 5.

ICP27 is necessary for JNK and p38 phosphorylation after HSV infection. (A) Replicate cultures of CV-1 cells were mock infected or infected with the indicated viruses as described in the text. Whole-cell lysates were prepared at 8 h postinfection, and accumulation of phosphorylated forms of p38 and JNK was determined by Western blot. Anisomycin was used as a positive control for p38 activation. Cells were treated with anisomycin (20 μg/ml) for 20 min prior to harvest. (B) Lysates prepared at 8 h postinfection were probed for the indicated viral proteins by Western blot.