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. 2024 Sep 13;16(9):1460. doi: 10.3390/v16091460

Table 1.

Overview table with the results of all samples for each DNA polymerase used. The serum, EDTA blood, lymph node (LN) and liver (li) samples are labeled with the respective animal number. The sequencing results are sorted according to their Ct value and divided by amplicon, where the samples are classified as follows: coverage depth higher than 10x (++), depth between 1 and 9 (+), no amplicon detected (-). Samples that were not amplified and sequenced are marked as not determined (ND).

qPCR Q5 DNA Polymerase Platinum SuperFi II DNA Polymerase
Ct Value B646L I73R-I329L B602L E183L B646L I73R-I329L B602L E183L
Serum85 16 ++ ++ ++ ++ + ++ - +
Serum90 16.1 ++ ++ ++ ++ ND ND ND ND
Serum86 17.4 ++ ++ ++ ++ ND ND ND ND
Serum87 17.4 ++ ++ ++ ++ ND ND ND ND
Serum88 17.5 ++ ++ ++ ++ ND ND ND ND
Serum89 18.2 ++ ++ ++ ++ ND ND ND ND
KWH 19.1 ++ ++ ++ ++ ++ ++ ++ ++
EDTA87 19.4 ++ ++ ++ ++ ND ND ND ND
EDTA90 19.4 ++ ++ ++ ++ ND ND ND ND
EDTA88 19.5 ++ ++ ++ ++ ND ND ND ND
EDTA89 19.6 ++ ++ ++ ++ ND ND ND ND
EDTA85 19.7 ++ ++ ++ ++ ++ ++ ++ ++
1819V 20.2 ++ ++ ++ ++ ++ ++ + ++
Perpignan 21.3 ++ ++ ++ ++ ++ ++ - ++
Malawi 21.6 ++ ++ ++ ++ ++ ++ - ++
Est75li 25.7 ++ ++ ++ - ++ ++ + +
Est68li 26.3 ++ ++ ++ - ++ ++ + +
Est75LN 29.9 ++ ++ + - ++ ++ - +
Est68LN 31.1 - - - - + ++ - +