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. 2024 Aug 29;16(9):1377. doi: 10.3390/v16091377

Figure 3.

Figure 3

AP3B1 ear domain is insufficient for inhibition of SARS-CoV-2. (A) A549 cells expressing human ACE2 were transfected with plasmids encoding full-length AP3B1 or the ear domain of AP3B1 only and infected with SARS-CoV-2 for 48 h. Release of infectious virus was measured by plaque assay. (B) Samples from (A) were analyzed by Western blotting for SARS-CoV-2 spike protein expression and (C) viability by MTT assay. Representative images shown from two independent experiments. (D) Immunofluorescence analysis of colocalization between full-length HA-AP3B1 (top panel) or HA-AP3B1 ear domain (bottom panel) and SARS-CoV-2 E (red). Scale bar represents 10 μM. Representative images shown from two independent experiments. White box depicts area shown in Inset. (E) Quantification of correlation coefficient from two independent experiments; data points represent individual cells. Error bars represent mean ± SD with statistics from one-way ANOVA with (A) Tukey’s post test or (B) Sidak’s post test. Data in (E) analyzed by two-way t-test. NS, not significant, *** p < 0.0005, **** p < 0.0001.