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AP3B1 is not interferon inducible. (A) A549 cells expressing human ACE2 were treated with 100 units/mL of IFNβ and analyzed for ISG15 and AP3B1 protein expression or (B) expression of AP3B1, IFITM1, and ISG15 mRNA normalized to 18s ribosomal RNA. Increased expression of ISG15 and IFITM1 confirms IFNβ stimulation of treated cells. Error bars represent mean ± SD; statistics performed by unpaired t-test; ns = not significant, ** p < 0.005, *** p < 0.0005.
