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. 2024 Sep 9;16(9):1436. doi: 10.3390/v16091436

Figure 2.

Figure 2

Viruses isolated from Patient 2 later during the infection have distinct temperature-dependent phenotypes compared to Day 0 virus. Comparisons: * (Day 0 to Day 134), $ (Day 0 to Day 137), # (Day 0 to Day 144). p values displayed as “ns” p > 0.05, one symbol is p < 0.05, two symbols are p < 0.01, three symbols are p < 0.001, and four symbols are p < 0.0001. (A,C): Representative images of plaques from each virus isolate at 33 °C and 37 °C, respectively, in Vero/TMPRSS2 cells. Scale bar = 10 mm. (B,D): Quantified plaque sizes, >500 (33 °C) and >390 (37 °C) plaques per virus accumulated from 3 independent experiments, one-way ordinary ANOVA with Bonferroni's multiple comparisons test (all comparisons to Day 0 isolate). (E,G): Growth curves showing virus replication on Vero/TMPRSS2 cells at 33 °C and 37 °C, respectively, the data are derived from 2 independent experiments with four wells per virus per experiment, standard deviation shown on error bars, and two-way repeated measures ANOVA with Bonferroni’s multiple comparisons test (all comparisons to Day 0 isolate). HPI = Hours Post Infection. (F,H): Total virus production on Vero/TMPRSS2 cells measured until peak tire at 33 °C (48 HPI peak) and 37 °C (36 HPI peak), respectively, one-way ordinary ANOVA with Bonferroni’s multiple comparisons test (all comparisons to Day 0 isolate). (I,K): Growth curves showing virus replication in hNECs at 33 °C and 37 °C, respectively, 3 independent experiments, standard deviation shown on error bars, and two-way repeated measures ANOVA with Bonferroni’s multiple comparisons test (all comparisons to Day 0 isolate). (J,L): Total virus production on hNECs measured until peak titer at 33 °C (120 HPI peak) and 37 °C (72 HPI peak), respectively, one-way ordinary ANOVA with Bonferroni’s multiple comparisons test (all comparisons to Day 0 isolate).