FIG. 5.

Increased CNS infiltration of inflammatory cells in mice following secondary infection with HI-BeAn. HI-BeAn or OVA-BeAn virus-infected mice received a secondary infection with HI-BeAn, OVA-BeAn, or ΔCla-BeAn at 14 days postinfection or were administered PBS. At 60 days postinfection, mice (n = 3) from all groups were anesthetized and perfused. The lumbar spinal cords, brain stems, and cerebellums of mice were removed and sectioned into 5-μm-thick slices. The sections were stained for the presence of CD4⁺ T cells and F4/80⁺ monocytes/macrophages in the lumbar spinal cord (a to p) and in the cerebellum and brain stem (q to x). Quantification of the immunohistochemistry was determined for representative sections by using ImageJ software. The number in the lower left corner of each panel indicates the percentage of positive pixels per area of the photomicrograph.